Journal: International Journal of Biological Sciences
Article Title: Integrin α1 promotes tumorigenicity and progressive capacity of colorectal cancer
doi: 10.7150/ijbs.37275
Figure Lengend Snippet: ITGA1 promotes the tumorigenicity in colorectal cancer (CRC) cells in vivo . 6-8 week-old female nude mice were subcutaneously injected with 200 μL Matrigel (BD) containing 2×10 6 virus-infected SW480 or NCM460 cells. The formation of tumor was evaluated at 3 weeks following the cell transplantation. A, images showed the sizes of tumors formed from NCM460 cells infected with LV-ITGA1 and LV-NC (left panel), and SW480 cells infected with LV-shITGA1 and LV-sh-NC (right panel). B, relevant quantification of the sizes (diameter) of tumors. These results showed that an ability of ITGA1 promotes CRC cell tumorigenicity. C, images showed Western blot of ITGA1 expression in the tumor tissues of nude mice. D, The relative levels of ITGA1 detected as evaluated by the relative density over β-actin in (C). E, representative images of Ki67 expression in the tumor tissues by immunohistochemistry. F, relevant quantification of the numbers of Ki67 positive cell. Compared with LV-NC or LV-sh-NC group, ** and *** represent p<0.01 and p <0.001, respectively. Data represented the mean ± SD from three independent triplicated experiments (n=6). Bars in A: 1.0 cm; Bars in E: 100 μm (up panel) and 50 μm (bottom panel), respectively.
Article Snippet: Paralleled sections were incubated with rabbit anti-human ITGA1 antibody (1:200, ab154845, Abcam, Cambridge, UK) and rabbit anti-human Ki67 antibody (1:200, 60065-1-Ig, Proteintech Group, Rosemont, IL, USA) overnight at 4 °C.
Techniques: In Vivo, Injection, Virus, Infection, Transplantation Assay, Western Blot, Expressing, Immunohistochemistry
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